A FATal AP-1 dimer switch in hepatosteatosis

Activator protein 1 (AP-1) is a dimeric transcription factor with important roles in specifying genetic and cellular programs. AP-1 refers to a collection of DNAbinding homoand heterodimers formed by members of the JUN (c-Jun, JunB, and JunD) and FOS (c-Fos, FosB, Fra-1, and Fra-2) families, as well as ATF and MAF proteins. AP-1 dimers recognize similar and specific sequence elements in the promoters and enhancers of target genes. The pool of AP-1 dimers is determined by the protein levels of its individual components and through upstream kinases. Various combinations of AP-1 dimers co-exist, and together with dimer-specific variation in target DNA sequence affinity determine the genes that are positively or negatively regulated by AP-1 in a given cell type and biological context. The liver is an essential metabolic organ, and understanding AP-1 function in the liver has been the focus of attention for many years. Gene inactivation and replacement experiments demonstrated an essential role of c-Jun in embryonic liver development and functional substitution of c-Jun by JunB or JunD. Furthermore, individual Jun and Fos genes appear dispensable for adult liver homeostasis, while having important and specific roles in the response of the liver to various stress conditions and in liver cancer. Importantly, the role of specific AP-1 dimers in adult liver function remained elusive. The functions of AP-1 were investigated in lipid metabolism of the liver, a central organ in the pathogenesis of obesity-associated metabolic diseases. Deregulation of hepatic lipid metabolism can result in lipid accumulation within hepatocytes, a condition called “fatty liver disease (FLD)” or hepatosteatosis. FLD is the most common liver disorder in industrialized countries and an important health concern. FLD often progresses to life-threatening conditions, such as liver inflammation (steatohepatitis), cirrhosis, and cancer. In the BXD population of inbred mouse strains, hepatic fra-1 was found reduced under high-fat diet (HFD), indicating that AP-1 might be a modulator of metabolic liver diseases. We next explored the role of AP-1 proteins in hepatic lipid metabolism using a non-exhaustive collection of AP-1 mouse mutants. These included broad and liverspecific loss-of-function mutants of AP-1 as well as recently generated Doxycycline (Dox)-switchable liver-specific gainof-function AP-1 monomer and dimer mutant mice. Strikingly, hepatocyterestricted Fra-1 expression in Fra-1 mice prevented and even reverted HFDinduced FLD by suppressing the transcription of the nuclear receptor PPARγ, a central regulator of lipid metabolism. Similarly, Fra-2 mice, expressing the closely related Fra-2 protein, also exhibited reduced PPARγ expression and signaling, leading to decreased expression of genes involved in hepatic fatty acid uptake and lipid droplet formation and reduced HFD-induced FLD. In contrast, ectopic c-Fos expression in murine livers increased the expression of PPARγ and PPARγ target genes. Consistently, chromatin immunoprecipitation experiments using mouse livers and human liver cell lines indicated that all Jun and Fos proteins can bind the proximal Pparg2 promoter. Notably, c-Fos and Fra-1/2 exerted antagonistic effects on transcription from a Pparg2 promoter reporter in human cell lines. An important implication of the combinatorial character of AP-1 is that functional redundancy between dimers could hinder the identification of AP-1 transcriptional targets in loss-of-function mouse models. Consistently, with the exception of Jund, genetic inactivation of individual Jun and Fos genes did not affect hepatic PPARγ expression and HFDinduced FLD. Importantly, changes in the levels of one AP-1 component can lead to qualitative and quantitative changes in other AP-1 dimers, which can affect gene expression, leading to phenotypic alterations that might not be directly related to the manipulated AP-1 monomer. To clarify how Fos proteins, which act as obligate heterodimers with Jun proteins, can antagonistically modulate Pparg2 expression, specific Fos-containing AP-1 dimers were expressed in Dox-switchable AP-1 mice. AP-1 monomers are joined by a flexible polypeptide tether to force specific pairing and insulate the forced dimer from other potential dimerizing partners. This “single-chain approach”, which is used for the first time in vivo, demonstrated that any Jun~c-Fos dimer, such as c-Jun~c-Fos, JunB~c-Fos, and JunD~cFos activated, whereas c-Jun~Fra-2 dimers inhibited PPARγ expression and signaling in the liver (Fig. 1). These data firmly establish AP-1 as a physiologically relevant regulator of PPARγ signaling and FLD and open potential therapeutic avenues for metabolic liver disease. We discovered an antagonism between specific AP-1 dimers on the same target gene, leading to opposing functional consequences and a striking, health-relevant metabolic phenotype in vivo. Previous studies in other organs,